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| Registros recuperados: 24 | |
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| Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães. |
| Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; CDNA; EST; Transcriptome. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008 |
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| Tambasco,Daniella Debenedetti; Alencar,Maurício Mello de; Coutinho,Luiz Lehmann; Tambasco,Antonio Junqueira; Tambasco,Marina Debenedetti; Regitano,Luciana Correia de Almeida. |
| No presente trabalho, 180 fêmeas da raça Nelore, provenientes de oito rebanhos, foram analisadas quanto aos marcadores microssatélites TEXAN15, BM1224 e CSFM50 e aos polimorfismos de comprimento de fragmentos de restrição (RFLP) nos locos k-caseína, beta-lactoglobulina e hormônio de crescimento (GH). Com exceção de GH, todos os marcadores foram polimórficos na amostra estudada. Os valores de heterozigosidade, diversidade gênica, conteúdo de informação polimórfica (PIC) e probabilidade de exclusão de paternidade (PE) foram estimados. Os maiores valores de PIC (0,685) e PE (0,521) foram obtidos para o marcador BM1224. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; DNA; Marcador; Microssatélite; Nelore; RFLP. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000400014 |
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| Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann. |
| Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008 |
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| Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva. |
| Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann. |
| A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001 |
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| Lopes,Mário Lúcio; Schmidt,Gilberto Silber; Coutinho,Luiz Lehmann. |
| Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube. Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100028 |
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| Ninov,Kerli; Ledur,Mônica Corrêa; Alves,Helena Javiel; Rosário,Millor Fernandes do; Nones,Kátia; Coutinho,Luiz Lehmann. |
| Leptin, a polypeptide hormone secreted mainly by adipose tissue, plays an important role in feed intake regulation, energy metabolism and reproduction in several species. Its function has been intensively studied in mammals; however, in birds limited information is available. The cDNA sequence for chicken leptin has been reported, and high hepatic expression levels of leptin were associated with fat deposition in selected bird lines. However, controversies still remain concerning to the chicken leptin gene and several authors failed to amplify this gene from genomic DNA or cDNA. In view of this controversy and the importance of this gene, the present study aimed to investigate the leptin gene in a population of birds developed by Embrapa Swine and Poultry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Fat deposition; Primer design. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200016 |
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| Kemenes,Paola Augusta; Regitano,Luciana Correia de Almeida; Rosa,Artur Jordão de Magalhães; Packer,Irineu Umberto; Razook,Alexander George; Figueiredo,Leopoldo Andrade de; Silva,Nirlei Aparecida; Etchegaray,Maria Antônia L.; Coutinho,Luiz Lehmann. |
| The genotypes for k-casein (<FONT FACE="Symbol">k</FONT>-CN), <FONT FACE="Symbol">b</FONT>-lactoglobulin (<FONT FACE="Symbol">b</FONT>-LG) and growth hormone (GH) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion in seven breeds of cattle (Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis). <FONT FACE="Symbol">k</FONT>-Casein had two alleles with the A allele occurring at a higher frequency in Bos indicus breeds (0.93, 0.92 and 0.91% for Gyr, Guzerá and Nelore, respectively). The <FONT FACE="Symbol">b</FONT>-lactoglobulin locus had two alleles in all of the breeds. European breeds had a higher frequency of the <FONT... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400012 |
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| Pinto,Luís Fernando Batista; Packer,Irineu Umberto; Ledur,Mônica Corrêa; Moura,Ana Silvia Alves Meira Tavares; Nones,Kátia; Coutinho,Luiz Lehmann. |
| This study aimed at mapping QTL (quantitative trait loci) using linear combinations of characteristics of economical interest in Gallus gallus. A total of 350 F2 chickens from an initial crossing among males from a broiler line (TT) with females from a layer line (CC) were used. It was conducted a QTL mapping in chromosomes of Gallus gallus (GGA1, GGA3, GGA5, GGA8, GGA11, and GGA13) for 20 performance and carcass traits. For detecting QTL, it was used the likelihood ratio test between a reduced model (including fixed effects of sex, hatch and random effect of infinitesimal genetic value) and a full model (including all the previous effects plus QTL effects). When original characterists were analyzed, that is, before the formation of linear combinations,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Canonical variables; Chickens; Genome. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001100016 |
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| Ziober,Iris Lamberti; Paião,Fernanda Gonzales; Marin,Silvana Regina Rockenbach; Marchi,Denis Fabricio; Binneck,Eliseu; Nepomuceno,Alexandre Lima; Coutinho,Luiz Lehmann; Shimokomaki,Massami. |
| Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ΑRYR; PSE; Chicken. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700029 |
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| Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p < 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007 |
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| Sousa Junior,Luis Paulo Batista; Meira,Ariana Nascimento; Azevedo,Hymerson Costa; Muniz,Evandro Nevez; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista. |
| Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aries; Candidate genes; Frequencies; Variants. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810 |
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| Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann. |
| T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012 |
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| Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida. |
| Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p < 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p < 0.05) at 0 cM from the centromere with an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011 |
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| Registros recuperados: 24 | |
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